Manuale d’uso / di manutenzione del prodotto 106Z del fabbricante Leica
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Leica Stereo- Fluorescence Systems User Manual.
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3 Contents Page Leica MZ10 F fluorescence stereomicroscope Description .............................................................. 4 Safety concept .......................................................... 4 Fluorescence applications .............
4 Description Fluorescence technique Some substances fluoresce when irradiated with short-wave light. This property is utilized in the fluorescence technique, in which certain structures and features which do not fluo- rescence can be tagged with a fluorescing dye.
5 Servicing and repairs – Repairs may only be carried out by Leica-trained service technicians or by technical personnel authorized to do so by the person in charge of the instrument – Only original Leica spare parts may be used – Unplug the power cable before opening the supply unit.
6 Applications Natural science Application Fluorescence filter set Anatomy •Monitoring of capillaryflow •GFP 1, GFP2 Biology •Gene expression inchick.
7 Industry Application Fluorescence filter set Electronics •Solder paste onSMDs •GFP 1, GFP2 •Epoxy resin onSMD plates •GFP 1, GFP.
8 Overview Leica MZ10 F: Its components and controls 1. Choice of stand and of focusing drive (manual or motor -driven) 2. Microscope carrier for Leica MZ10 F 3a. Leica MZ10 F optics carrier with third beam path 3b. Integrated FLUOIII filter system 3c.
9 Filter sets Designation Excitation filter Barrier filter GFP fluorescence GFP1 425/60 nm 480 nm GFP Plus fluorescence GFP2 480/40 nm 510 nm GFP plant fluorescence GFP3 470/40 nm 525/50 UV fluorescen.
10 Leica MZ10 F Assembly 1. Incident- or transmitted-light base 2a. Manual focusing drive, or 2b. Motorized focusing drive 3. Microscope carrier for Leica MZ10 F 4. Leica MZ10 F optics carrier with FLUOIII™ filter system 5a. Lamp housing 106Z 5b. Stray-light protection 6.
11 Leica MZ10 F Focusing drive ➜ base of stand ▶ Connect manually-operated or motor -driven focusing drive, complete with column, to base, as described in user manual M2-105-0EN. Before using the motor focus, you must read the safety directions in the accompanying user manual M2-267- 104.
12 Assembly Lamp housing The Leica 106Z lamp housing is equipped. For information about the supply units for the 106Z lamp housing, see page 14. Model 105Z lamp housings, if available, can also be used.
13 ▶ Using the focusing knob, displace the collector: • Duringthis movement, the collector must not touch the flexible power cable. If necessary , bend the cable away ▶ Carefully .
14 Assembly Supply unit for lamp housing 106Z with 50W mercury-vapour burner 1. On/off switch 2. Running-time meter 3. Selector for L1/L2 burners 4. Fuse 5. Selectorfor 50Hz/60Hz 6. Connector for lamp 7. Power cable The supply unit is intended for power supplies above 220V .
15 3 1 2 4 5 6 Hg50W Hg 100W Adjusting the mercury vapour lamp This adjustment is important for obtaining a uniform light spot and good-quality fluorescence.
Dummy filter carrier In the dummy filter carrier there are two openings for the observation beam paths instead of the barrier filters. The third path has been closed. Use the dummy filter carrier: – if you are using less than four sets of filters – if you are working without the fluorescence illuminator for periods of up to 15 seconds.
17 Use MZ10 F: Bringing the specimen into focus and observing it Observe the safety directions on page 4 and the recom- mendations on page 5 regarding the use of the mercu- ry-vapour lamp. Changing filters In the rapid filter changer , all four positions must be occupied, either with filter sets or with dummy filter carriers (see page 16).
18 Overview Outfit with stereo-fluorescence module The components 1 Choice of stand and of focusing drive (manual or motor -driven) 2 Microscope carrier 3 Leica MS5, MZ6, MZ7.
19 Filter sets Excitation Dichromatic Barrier filters beam splitters filters GFP fluorescence 425/60nm 470nm GG475 GFP-Plus fluorescence 480/40nm 505nm LP 510nm LP GFP-Plant 470/40nm 495nm 525/50nm UV.
20 Assembly Stereo-fluorescence module 1 Incident- or transmitted-light base 2a Manual focusing drive, or 2b Motorized focusing drive 3 Microscope carrier 4 Choice of optics carrier 5 Fluorescence mod.
21 Important: With the illuminator on, insert the filterhol- der with diaphragm into slide-in unit 3 to avoid being suddenly exposed to excessively bright light (page 22). ▶ Remove the binocular tube. ▶ Loosen the 3 socket-head screws. ▶ Remove the cover .
22 Assembly Stereo-fluorescence module UV-light excluder ➜ side-faced column Always position the UV-light excluder so that the user never looks directly at the light spot (page 5). ▶ Using the clamping screw , secure the clamp on the side- faced column, beneath the focusing drive.
23 Use Stereo-fluorescence module Focusing and observing Observe the safety regulations on page 4 and note the recommendations on page 5 for using the mercury- vapour burner .
L ei c a M i c r os y s te ms – th e b r a n d f o r o u ts tan d i n g p r od u c ts Leica Microsystems – an international company with a strong network of customer services. Australia: Gladesville T el. +61 2 9879 9700 Fax +61 2 9817 8358 Austria: Vienna T el.
Un punto importante, dopo l’acquisto del dispositivo (o anche prima di acquisto) è quello di leggere il manuale. Dobbiamo farlo per diversi motivi semplici:
Se non hai ancora comprato il Leica 106Z è un buon momento per familiarizzare con i dati di base del prodotto. Prime consultare le pagine iniziali del manuale d’uso, che si trova al di sopra. Dovresti trovare lì i dati tecnici più importanti del Leica 106Z - in questo modo è possibile verificare se l’apparecchio soddisfa le tue esigenze. Esplorando le pagine segenti del manuali d’uso Leica 106Z imparerai tutte le caratteristiche del prodotto e le informazioni sul suo funzionamento. Le informazioni sul Leica 106Z ti aiuteranno sicuramente a prendere una decisione relativa all’acquisto.
In una situazione in cui hai già il Leica 106Z, ma non hai ancora letto il manuale d’uso, dovresti farlo per le ragioni sopra descritte. Saprai quindi se hai correttamente usato le funzioni disponibili, e se hai commesso errori che possono ridurre la durata di vita del Leica 106Z.
Tuttavia, uno dei ruoli più importanti per l’utente svolti dal manuale d’uso è quello di aiutare a risolvere i problemi con il Leica 106Z. Quasi sempre, ci troverai Troubleshooting, cioè i guasti più frequenti e malfunzionamenti del dispositivo Leica 106Z insieme con le istruzioni su come risolverli. Anche se non si riesci a risolvere il problema, il manuale d’uso ti mostrerà il percorso di ulteriori procedimenti – il contatto con il centro servizio clienti o il servizio più vicino.
