Manuale d’uso / di manutenzione del prodotto RPN2510E del fabbricante GE
Vai alla pagina of 22
GE Healthcare Amersham Hybridization oven/shak er Pr oduct User Manual Codes: RPN2510E RPN2511E RPN2512E.
RPN2510EUM Pagefinder Rev C 06/2007 2 Page finder 1. Legal 3 2. Introduction 4 3. Safety warnings and precautions 5 3.1. Electrical Installation 5 4. Specification 6 5. Setting up the Hybridization O ven/Shak er 7 5.1. Setting the oven temperature 7 5.
1. Legal GE, imagination at work and GE monogram are trademarks of General Electric Company. Amersham, Alkphos Direct , ECF , ECL , ECL Plus, ECL Advance, Hybond, Hypercassette, Hyperfilm, Hyperscreen, Hyper torch, Megaprime, Nick, Rapid- hyb, Ready-To-Go , Rediprime, Sensitive and Trackertape are trademarks of GE Healthcare companies.
2. Intr oduction One of the most widely used techniques in the molecular biolog y field is the immobilization of DNA and RNA onto a solid suppor t membrane and subsequent hybridizations with a specific single stranded probe, labeled to facilitate its detection.
3. Safety w arnings and pr ecautions If the equipment is not used in the manner described in this manual the protection provided might be impaired This equipment is designed to operate under the follo.
4. Specification Overall dimensions Height: 17” (43.5 cm) Depth: 15” (38.0 cm) Width: 15” (38.0 cm) Oven dimensions Height: 8” (20.0 cm) Depth: 9” (23.0 cm) Width: 10” (25.0 cm) Weight: 24 kg Capacity (nominal): 18 litres T emperature range: Ambient plus 5°C–80°C T emperature precision: +/- 0.
5. Setting up the Hybridization O ven/Shaker RPN2510EUM Chapter 5 Rev C 06/2007 7 Fig 1. Diagram of instrument control panel Rotisserie/shaker controls 1. Rotisserie/shaker ON/OFF button 2. Rotisserie/shaker speed selector dial 3. Motor on indicator Temperatur e controls 4.
4. Place the rotisserie into the oven onto the rotation mechanism, ensuring that the serrated bands at either end of the rotisserie locate onto the steel cogs of the rotation mechanism at the rear of the oven. (B on figure 2). The plastic flanges of the r otisserie locate on to the small wheels on the oven floor .
RPN2510EUM Chapter 6 Rev C 06/2007 9 6. Hybridization using the platform shaker The Hybridization Oven/Shaker is compatible with the hybridization technologies available from GE Healthcare.
RPN2510EUM Chapter 7 Rev C 06/2007 10 Several advantages are associated with performing hybridizations in bottles, namely those of safety and economy as outlined in the introduction. However , the use of bottles for hybridizations and washing procedures requires certain adaptations to standard protocols.
RPN2510EUM Chapter 7 Rev C 06/2007 11 4. Prehybridize the membrane for the specified length of time at a rotisserie speed of 4 rpm. 5. Following prehybridization, turn off the rotisserie, remove the rotisserie from the oven.
RPN2510EUM Chapter 8 Rev C 06/2007 12 8. Maintenance/car e/cleaning of the oven/shak er The GE Healthcare Hybridization Oven/Shaker is designed to provide trouble-free operation. The base of the oven and the shaker tray act as a spills tray and will contain any spillage that occurs during hybridization and washing procedur es.
RPN2510EUM Chapter 9 Rev C 06/2007 13 Symptom 1. Membrane curling up in hybridization bottle 2. High background 3. Weak signal 4. Pat chy backgrounds 5. High background around edge of membrane Cause 1.1. Incorrect orientation of bottle in rotisserie 2.
Appendix 1. Pr oducts for electr ophor esis DNA Markers Precise Sizing Digest of Natural DNAs 50 Base-pair 100 Base-pair KiloBase DRigest III ladder ladder DNA marker 27-4060-01 27-4005-01 27-4007-01 .
Appendix 2. Hybond membranes for nucleic acid applications Size Pack size Hybond N+ Hybond XL Hybond N Hybond NX Hybond ECL Hybond C Extra Hybond P 82 mm 50 discs RPN82B RPN82S RPN82N RPN82T RPN82D RP.
Appendix 3. Radioactive labeling systems Labelling T echnology Nucleotide Amount of Labelling Probe specific Recommended Product system template time activity application code (dpm/µg) Rediprime ™ .
Appendix 4. Non-radioactive labeling and detection systems Labelling and detection Sensitivity Time from Duration of Strip before Quantification Recommended application system hybridization light output re-pr obing to detection AlkPhos Direct (RPN3690) 0.
Appendix 5. Pr oducts for autoradiography and chemiluminscent detection Hyperfilm – high performance autoradiography films Product description size number sheets code Hyperfilm ECL 5 × 7 inches 50 .
Appendix 6. Hybridization buffer Product name Pack size Code Rapid-Hyb buffer Rate enhanced hybridization buffer 125 ml RPN1635 for use with radiolabelled nucleic acid probes 500 m RPN1636 RPN2510EUM .
Appendix 7: Radiation safety pr oducts Product Description Product Dimensions Quantity Code Number CDC storage box 240 × 140 × 130 mm 1 box RPN2032 Pipette guards Beta pipette guard, Gilson P20/100 .
21.
RPN2510EUM Rev C 06/2007 imagination at work GE Healthcare offices: GE Healthcare Bio-Sciences AB Björkgatan 30, 751 84 Uppsala, Sweden GE Healthcare Europe GmbH Munzinger Strasse 5, D-79111 Freibur g, Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P .
Un punto importante, dopo l’acquisto del dispositivo (o anche prima di acquisto) è quello di leggere il manuale. Dobbiamo farlo per diversi motivi semplici:
Se non hai ancora comprato il GE RPN2510E è un buon momento per familiarizzare con i dati di base del prodotto. Prime consultare le pagine iniziali del manuale d’uso, che si trova al di sopra. Dovresti trovare lì i dati tecnici più importanti del GE RPN2510E - in questo modo è possibile verificare se l’apparecchio soddisfa le tue esigenze. Esplorando le pagine segenti del manuali d’uso GE RPN2510E imparerai tutte le caratteristiche del prodotto e le informazioni sul suo funzionamento. Le informazioni sul GE RPN2510E ti aiuteranno sicuramente a prendere una decisione relativa all’acquisto.
In una situazione in cui hai già il GE RPN2510E, ma non hai ancora letto il manuale d’uso, dovresti farlo per le ragioni sopra descritte. Saprai quindi se hai correttamente usato le funzioni disponibili, e se hai commesso errori che possono ridurre la durata di vita del GE RPN2510E.
Tuttavia, uno dei ruoli più importanti per l’utente svolti dal manuale d’uso è quello di aiutare a risolvere i problemi con il GE RPN2510E. Quasi sempre, ci troverai Troubleshooting, cioè i guasti più frequenti e malfunzionamenti del dispositivo GE RPN2510E insieme con le istruzioni su come risolverli. Anche se non si riesci a risolvere il problema, il manuale d’uso ti mostrerà il percorso di ulteriori procedimenti – il contatto con il centro servizio clienti o il servizio più vicino.
